LIFT of biomolecules

Laser printing of DNA and protein microarrays

 

 

 

 

 

 

 

Laser printing of DNA and protein microarrays. The transfer of biomaterials, such as proteins, nucleic acids and oligonecleotides has a crucial role in the development of microarrays, biosensors, medical sensors and lab-on-chip devices. The printing of microarrays by liquid phase LIFT offers high spatial resolution, contact less approach and minimization of the biological material waste.

People: Christos Boutopoulos, Postdoctoral Fellow, École Polytechnique de Montréal Ioanna Zergioti, Assis. Professor, NTUA

 

Laser printing of Thylakoids for amperometric biosensors

 

 

 

 

 

 

 

 

One of the most crucial steps for the photosynthetic amperometric sensors preparation is the immobilization of the photosynthetic material onto the electrodes surface. LIFT has been proven an advanced tool for direct immobilization of photosynthetic material onto low cost non-functionalized Au screen printed electrodes. The high kinetic energy of the transferred material enhances physical adsorption onto the electrode surface and results in high photocurrent activity by using extremely low quantities of photosynthetic material (thylakoid membranes).

People: Christos Boutopoulos, Postdoctoral Fellow, École Polytechnique de Montréal Dr. Lefteris Touloupakis, Biosensor, Rome Prof. Maria Tereza Giardi, CNR, Rome Ioanna Zergioti, Assis. Professor, NTUA Funding FP7, ICT "Flexible, Organic and Large Area Electronics "e-LIFT"(2010-2012)

 

Laser printing of oligonucleotides onto micro-membrane arrays for DNA mutations detection

 

The accuracy and the flexibility of LIFT technique enabled precise transfer of oligonecleotides probe sequences, CD 19 normal and CD 19 mutated, onto different membranes of a capacitive micro-array. The micro-membrane arrays have been tested with a complementary oligonucleotide to the immobilized probe sequences at a concentration of 36 nM and measured significant real time response during the hybridization process.

People: Christos Boutopoulos, Postdoctoral Fellow, École Polytechnique de Montréal Marianneza Chatzipetrou, PhD student , NTUA Ioanna Zergioti, Assis. Professor, NTUA Collaborators Dr. S. Chatzandroulis, (Institute of Microelectronics, NCSR Demokritos) Dr. V. Tsouti, (Institute of Microelectronics, NCSR Demokritos) Funding National project Corallia,( 2009-2012) “Labonchip”